Tell us about yourself (Who are you? Where are you from? What is your educational background?)
Name: Alejandro Belanche
Nationality: Spain
Education: Veterinary and PhD on Ruminant Nutrition and rumen microbiology
Working place: EEZ-CSIC, Granada, Spain
Contact: a.belanche@csic.es
What is your area of expertise?
Ruminant Nutrition
Rumen Microbiology
R&D programs and technology transfer to the sector
Where does your focus lie within your current project(s)? (related to methane)
iSAGE project (H2020). Innovation for sustainable sheep and goat production in Europe.
RumenStability project (FACE-JPI): Early life interventions to decrease methane emissions in ruminants.
What would be the added value for you of joining an international researchers network? (such as the
METHAGENE network)
Network with international researchers
Up-dates in methane mitigation strategies
What and/or who inspired you to make a career in science?
Veterinary vocation and rural development.
What do you enjoy most about your work?
Designing and conducting experiments. Interaction and knowledge transfer to the ruminant sector.
What is your most stand-out or surprising moment in science so far?
Main Publications:
Belanche A., Erroa I.R., Balcells J., Calleja L. 2010 “Use of quantitative real-time PCR to assess the
in vitro survival of specific DNA gene sequences of rumen microbes under simulated abomasum conditions“
Journal of Animal Physiology and Animal Nutrition. 94, pp 204-211.
Belanche A., Balcells J., de la Fuente G., Yañez-Ruíz D.R., Fondevila M. and Calleja L. 2010.
Description of development of rumen ecosystem by PCR assay in milk-fed, weaned and finished lambs in an intensive
fattening system. Journal of Animal Physiology and Animal Nutrition, 94: 648-658.
Belanche A., de la Fuente G., Yáñez-Ruiz,. D.R., Newbold D.J., Calleja, L. and Balcells,
J. (2011) Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs
and its potential use as microbial markers. Journal of Animal Sciences. 89(9): 2812-2816.
Belanche A, Abecia L, Holtrop G, Guada JA, Castrillo C, de la Fuente G & Balcells J (2011) Study
of the effect of presence or absence of protozoa on rumen fermentation and microbial protein contribution to the
chyme. Journal of Animal Science 89(12): 4163-4174.
de la Fuente, G; Belanche, A; Abecia, L; Dehority, B.A; Fondevila, M. (2009). “Rumen protozoal
diversity in the Spanish Ibex (Capra pyrenaica hispanica) as compared with domestic goats (Capra hircus)”.
European Journal of Protistology, 45, pp. 112-120.
G. de la Fuente; A. Belanche; M. Fondevila. (2008) “Estudio in vitro de la fermentación ruminal
de carbohidratos en cabra montés“ (2008). Archivos de Zootecnia 58 (223) pp. 357-362.
Belanche A, Doreau M, Edwards JE, Moorby JM, Pinloche E, Newbold CJ. (2012) Shifts in the Rumen
Microbiota Due to the Type of Carbohydrate and Level of Protein Ingested by Dairy Cattle are Associated with
Rumen Fermentation. Journal of Nutrition 142: 1684-1692.
Belanche A, de la Fuente G, Moorby JM, Newbold CJ. (2012) Bacterial protein degradation by different
rumen protozoal groups. Journal of Animals Sciences 90(12):4495-4504.
Belanche A, de la Fuente G, Pinloche E, Newbold CJ, Balcells J. (2012) Effect of diet and absence of
protozoa on the rumen microbial community and on the representativeness of bacterial fractions used in the
determination of microbial protein synthesis.. Journal of Animal Sciences 90(11):1-13.
Belenguer, A. Abecia, L. Belanche, A. Milne, E., Balcells, J. (2012) Effect of carbohydrate source on
microbial nitrogen recycling in growing rabbits. Livestock Sciences 150: 94-101.
Belanche A, M.R.F. Lee, J.M. Moorby and C.J. Newbold (2013). Comparison of ryegrass and red clover on
the fermentation pattern, microbial community and efficiency of diet utilization in the rumen simulation technique
(Rusitec). Animal Production Sciences 53 (10) 1052-1064.
Guadagnin M, F. Tagliapietra, M. Cattani, S. Schiavon, A. Belanche, C.J. Newbold, L. Bailoni (2013)
In vitro rumen fermentation and microbial yield of high- or low-protein diets containing ground soybean seeds or
home-made rapeseed expellers. Canadian Journal of Animal Sciences. 93(3): 363-371.
Belanche A., Weisbjerg M. R., Allison G. G., Newbold C. J. and Moorby J. M. (2013) Estimation of feed
crude protein concentration and rumen degradability by Fourier-transform infrared spectroscopy (FTIR). J. Dairy
Sci. 96 (12) 7867-7880.
Belanche A., Weisbjerg M. R., Allison G. G., Newbold C. J. and Moorby J. M. (2014) Measurement of
rumen dry matter and neutral detergent fiber degradability of feeds by Fourier-transform infrared spectroscopy.
J. Dairy Sci. 97:2361-2375.
De la Fuente G, Belanche A, Girwood SE, Pinloche E, Wilkinson T, Newbold CJ. (2014) Pros and Cons of
Ion-Torrent Next Generation Sequencing versus Terminal Restriction Fragment Lenght Polymorphism T-RFLP for Studing
the Rumen Bacterial Community. PlosOne 9 (7): 1-13.
Belanche A, de la Fuente G, Newbold C.J. (2014). Study of methanogen communities associated with
different rumen protozoal populations. FEMS Microbiology Ecology 90, 663-377.
Belanche A, de la Fuente G, Newbold C.J. (2015). Effect of progressive inoculation of fauna-free sheep
with holotrich protozoa and total-fauna on rumen fermentation, microbial diversity and methane emissions.
FEMS Microbiology Ecology 91: 1-10.
Belanche A, Ramos-Morales E, Newbold C J (2015) In vitro screening of natural feed additives from
crustaceans, diatoms, seaweeds and plant extracts to manipulate rumen fermentation. Journal of the Sciences of
the Food and Agriculture 96: 3069-3078
Newbold C J, de la Fuente G, Belanche A, Ramos-Morales E and McEwan N R 2015. The role of ciliate
protozoa in the rumen: a review. Research Topic: Engineering rumen metabolic pathways: where we are, and where
are we heading. Frontiers in Microbiology 6(1313): 1-13. All authors equally contributed.
Belanche A, Pinloche E, Preskett D, Newbold CJ (2015). Effects and mode of action of chitosan and ivy
fruit saponins on the microbiome, fermentation and methanogenesis in the rumen simulation technique. FEMS
Microbiology Ecology. 92:(1) 1-13.
Belanche A, Jones E, Parveen I, Newbold CJ (2016). A metagenomics approach to evaluate the impact of
dietary supplementation with Ascophyllum nodosum or Laminaria digitata on rumen function in rusitec fermenters.
Frontiers in Microbiology 7(299): 1-14.
Belanche A, Kingston-Smith, AH, Newbold CJ (2016). An integrated multi-omics approach reveals the
effects of supplementing grass and grass hay with vitamin E on the rumen microbiome and its function Frontiers in
Microbiology 7(905):1-17.